EASY Isotyping and IgG Quantification ELISAs

New! EZ ELISA for Isotyping  

Mouse Immunoglobulin EASY Isotyping Kit

The Mouse IgG EASY Isotyping Kit provides a simple, rapid method (2-step ELISA) to characterize mouse monoclonal antibody isotypes in cell culture supernatants or purified antibody preparations.
Specificity: The method enables the detection of Light chains (Kappa and Lambda), and Heavy chains (Gamma 1, Gamma 2a, Gamma 2b, Gamma 3, and Mu) of mouse immunoglobulin.

The kit includes ready-to-use reagents necessary to analyze 12 samples in less than 30 minutes. Buffer solutions are color coded in order to simplify pipetting steps. The method employs the quantitative sandwich enzyme immunoassay technique. Anti-mouse antibodies specific to each of the common light and heavy chains are pre-coated in the wells. Samples are pipetted into microwells and Ig present in the sample are bound by the capture antibody. Then, a HRP (horseradish peroxidase) conjugated anti-mouse IgG (H+L) antibody is pipetted and incubated simultaneously with samples. After washing microwells in order to remove any non specific binding, the ready to use substrate solution (TMB) is added to microwells and color develops if the specific immunoglobulin is present in the sample. Color development is then stopped by addition of stop solution. Absorbance is measured at 450 nm.

Cat. No. EZ Mouse Isotyping ELISA Quantity  Price
IS001A Mouse Immunoglobulin EASY Isotyping Test Kit 1 x 12 tests  $    250
IS001B Mouse Immunoglobulin EASY Isotyping Test Kit 5 x 12 tests  $    575

Typical Result:

ISOresult

Characterization of an IgG1 antibody: yellow color is observed in well A (corresponding to IgG1 Heavy chain), well F (corresponding to Kappa Light chain), and well H (for the positive control H+L). The well H has to be positive in order to validate the method.


 

New! EZ ELISAs for IgG Quantification

Features

- No reagent preparation
- User-friendly system and easy handling
- Divisible strips of 8 wells
- Results in 30 minutes, only one incubation step
- Color-coded reagents eliminate operator error
- A wide dynamic range (from 4 to 2000 ng/ml)

Applications

- Monoclonal antibodies production monitoring
- Screening of therapeutic antibodies
- Selection of high-producing clones
- Vectors validation for transfection
- Contaminants detection

Cat. No. EZ IgG Quantification ELISAs Quantity  Price
IS002A Human IgG Easy Quantification ELISA Kit 1 x 96 tests  $    495
IS002B Human IgG Easy Quantification ELISA Kit 10 x 96 tests  $ 2,425
IS003A Mouse IgG Easy Quantification ELISA Kit 1 x 96 tests  $    495
IS003B Mouse IgG Easy Quantification ELISA Kit 10 x 96 tests  $ 2,425
IS004A Rat IgG Easy Quantification ELISA Kit 1 x 96 tests  $    495
IS004B Rat IgG Easy Quantification ELISA Kit 10 x 96 tests  $ 2,425
IS005 Bovine IgG Easy Quantification ELISA Kit 1 x 96 tests  $    495

The simple EZ ELISA procedure is outlined in the steps below.

Pre-coated plate with the Mouse Anti IgG capture antibody:

ISO1

Step 1:  Standards are pipetted into the microwells. The range of 6 standards is applied to the plate wells.

ISO2

 

The diluted samples are pipetted into wells.

ISOSamples

 

The secondary antibody, HRP-conjugated anti-mouse IgG antibody is immediately pipetted into microwells.

ISOHRPAB

 

The plate is incubated for 15 minutes at room temperature, and then washed.

After washing, substrate solution (TMB) is added to the microwells.

ISOTMB

 

After a 10 minute incubation at RT, Stop Solution is added to the wells.

ISOStop

The ELISA is completed in only 30 minutes.

 

Measurement of absorbance and results analysis:

ISOAnalysis

Typical Data

ISOData

For a limited time receive a free Sport Water Bottle with your first order of EZ ELISA Mouse Isotyping Kits or any of our new Human, Mouse, Rat, or Bovine IgG Quantification Kits. Mention the code EZ ELISA when you place your order.

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